PandaPure® offers you next-level experiences of protein research, by eliminating the need for traditional chromatography, buffer exchange and protease treatments, and improving expression of many difficult proteins. Now, you can obtain tag-free proteins with the fewest operations, reagents and the least effort.
purification
Easier. Faster. Simpler.
Cells, not human, doing most of the works
expression
Express your interests.
PandaPure makes more proteins expressible in E. coli.
efficiency
Recover up to 70% yield of expressed target proteins.
efficiency
Get >95%-purity native proteins < 1 day.
Throughput
96 in Parallel.
Suitable for multi-well plates and automation
scalability
Scaling. Up and down.
From μL, To liters.
scope of applications
PandaPure makes proteins more accessible at higher yields than traditional E. coli.1
Synthetic organelles in PandaPure provide a distinct environment for protein folding and maturation, and also protect hosts from potential toxicity of expressed proteins, leading not only a broadened scope of applicable proteins in E. coli, but also higher production yield in general.
See case studies of PandaPured proteins, and join us to explore the new limit:
Superfolder GFP
Proinsulin, human insulin precursor
DpnI, restriction enzyme
Getting started.
PandaPure requires engineered DNAs to program your bacterial hosts, and reagents to operate the downstream processing workflow. Here we offer Predefined Kits, separate plasmids and reagents, and additional Construct Service. Place an order >>
PandaPure®️ Toolkit
Predefined ToolKits for common applications
from lab-scale production to high-throughput screening Read more
PandaPure®️ Original Plasmids
Genetic elements that make cells purify proteins
Plasmids for synthetic organelles, expression vectors, controls, in various options. Read more
PandaPure®️ Protein Reagent
All reactions in one reagent, including tag removal and protein recovery.
from $64.49
PandaPure®️ Lysis Reagent
Easy-to-use, gentle lysis for bacteria hosts.
from $135.00
Construct Service
Build your strains for PandaPure effortlessly.
Get ready-to-use DNA at a competitive price from US$0.1/bp2 Read more
PandaPure®️ License
MTA & Commercial Licenes
For startup and industrial users to exploit PandaPure for commercial purposes. Read more
Take a closer look.
Cells are isolating your proteins.
Now, protein purification is
Easier than ever.
PandaPure is designed to minimize the complexity of protein purification, extracting pure proteins with only one step of pipetting and centrifugation. The hands-on workload becomes negligible. Now, anyone who had basic molecular biology training can apply protein purification in their research.
Save up to $300 per 1mg protein3
Up to 10x cheaper
PandaPure offers higher yield, higher purity, tag removal, high throughput enabling and more, only at the cost of simplistic his-tag affinity purification.
Sciences behind PandaPure
Synthetic sorting
PandaPure® Protein Constructs encode proteins of interest (POI), and also synthetic organelles with cognate receptors. Thus, synthetic organelles can selectively sort, purify, and store proteins of interest.
Protein recovery
In a single reaction under mild conditions
PandaPure® Protein Reagent induces self-cleavage of signal tags, thus enables the extraction of tag-free proteins in a single reaction.
Cited reference
1. About synthetic organelle: Guo, Haotian, et al. "Spatial engineering of E. coli with addressable phase-separated RNAs."Cell 185.20 (2022): 3823-3837. 2. About PandaPure: preprints in preparation, coming soon.
"Traditional E. coli" refers to commonly-used, commercially-available bacteria strains such as BL21(DE3), and expression vectors such as pET-28a(+). For some proteins, other strain engineering methods to modify genome and plasmid sequences may lead to optimization in protein expression.
Gene synthesis prices are considered "competitive" based on publicly listed pricing from DNA and gene synthesis suppliers as of Dec 15, 2023. Turnaround times may vary due to complexity of the order, and shipments.
Savings based on publicly listed pricing as of Dec 15, 2023 of products from Thermo Fisher Scientific and Cytiva, in order to process 1 mg protein. Loss of proteins in each step is not counted. The exact saving can vary according to the differences among experiments, and may be even higher than what we estimate here.